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Bunky ženských vaječníkov vytvorené v laboratóriu môžu znamenať revolúciu v liečbe plodnosti a dokonca aj odvrátiť menopauzu

Obrazok Prvé bunky ženských vaječníkov, ktoré boli vytvorené v laboratóriu z kmeňových buniek by mohli byť oplodnené ešte tohto roku. To by znamenalo revolúciu v liečbe plodnosti a dokonca by mohol viesť k odvráteniu menopauzy u starších žien.

Vedci sa chystajú požiadať o povolenie britský orgán dohľadu, aby mohli oplodniť vajíčka ako súčasť série testov, ktorých cieľom je vyprodukovať prakticky neobmedzený počet ľudských vajíčok. Môže to znamenať prelom v pomoci neplodným ženám, a taktiež to, že ženy by mohli byť plodné aj v neskoršom veku podobne ako muži.

Produkcia ľudských vajíčok z kmeňových buniek by tiež znamenala možnosť doplňovať vaječníky starších žien, ktoré by tak nemuseli čeliť zdravotným problémom spojeným s vekom a menopauzou, od osteoporózy až po ochorenia srdca. Niektorí vedci dokonca navrhujú možnosť vyprodukovať „elixír mladosti“ pre ženy, vďaka ktorému by bola odstránená menopauza a staršie ženy by si tak udržali rovnaký zdravotný stav ako mali v mladšom veku. Výskumníci z Univerzity v Edinburgu spolu s tímom harvardskej lekárskej fakulty v Bostone pracujú na získaní svetového prvenstva vo vyprodukovaní zrelých ľudských vajíčok z kmeňových buniek izolovane od tkaniva vaječníkov.

Doteraz bolo možné izolovať iba relatívne malý počet zrelých buniek vajíčok z vaječníkov žien, ktoré boli stimulované hormónmi. To znamenalo akútny nedostatok ľudských vajíčok (oocytov) potrebných ako pre asistovanú reprodukciu, tak aj pre vedecký výskum. Vedci chcú oplodniť laboratórne vytvorené vajíčka ľudskými spermiami aby potvrdili ich životaschopnosť. Všetky vzniknuté embryá budú pozorovať 14 dní (zákonný limit) aby videli či sa vyvíjajú normálne. Tieto embryá budú považované za pokusný materiál a preto nebudú prenesené do maternice ženy. Po 14 dňoch budú buď zmrazené alebo bude umožnené usmrtiť ich.

Evelyn Telferová, reprodukčná biologička z Edinburskej univerzity, sa už neformálne obrátila na Orgán pre dohľad nad oplodňovaním a embryológiou (Human Fertilisation and Embryology Authority, HFEA) a oficiálnu žiadosť o povolenie predloží do niekoľkých týždňov. „Chceme požiadať o povolenie na výskum, aby sme mohli vykonať oplodnenie oocytov vypestovaných in vitro v rámci IVF jednotky v priestoroch Univerzitnej nemocnice v Edinburgu,“ hovorí doktorka Telferová. Podľa jej slov by sa oplodnenie mohlo uskutočniť už tento rok. Profesor Richard Anderson z centra pre reprodukčné zdravie, ktorý bude na čele klinickej časti výskumu, povedal: „Cieľom bude ukázať, že vajíčka, ktoré vypestujeme in vitro sú schopné vytvoriť embryo.

Produkovanie nelimitovaného množstva ľudských vajíčok a vyhliadka odvrátenia menopauzy je možná vďaka sérii objavov a výskumov vedených profesorom Jonathanom Tillym z Harvardu. V roku 2004 ohromil svet reprodukčnej biológie tým, že vo vaječníkoch myší identifikoval aktívne kmeňové bunky, ktoré sa zdajú byť schopné doplňovať vajíčka po celý život. Polstoročia zaužívaná dogma reprodukčnej biológie uvádza, že ženy sa narodia s určitým počtom vajíčok, o ktoré počas života už len prichádzajú až do nástupu menopauzy. Podľa profesora Tillyho je presvedčenie, že ženy majú pri narodení fixný počet vajíčok nesprávny. Hovorí, že v skutočnosti sa v dospelosti vaječníky podobajú semenníkom čo sa týka ich schopnosti tvoriť nové zárodočné bunky – špeciálne bunky, z ktorých vznikajú spermie a vajíčka. Dodáva, že za posledných 50 rokov všetky klinické výskumy a veda ako taká boli založené na presvedčení, že vo vaječníkoch sa nachádza fixný počet vajíčok, ktoré nie je možné obnoviť, doplniť ani nahradiť.
Minulý mesiac profesor Tilly publikoval priekopnícky výskum, podľa ktorého v ľudských vaječníkoch existujú kmeňové bunky, ktoré môžu byť v laboratórnych podmienkach stimulované  k tomu, aby sa vyvinuli na nezrelé bunky vajíčok.

Doktorka Telferová, s ktorou spolupracuje, bola k jeho výskumu spočiatku skeptická. Ona sama bola v Edinburgu priekopníčkou techniky, vďaka ktorej sa nezrelé bunky vajíčok vyvinú do „dozretého“ štádia, v ktorom môžu byť oplodnené. „Bolo zábavné pracovať s ňou vzhľadom na to, že pred rokmi bola jednou z najvýraznejších kritičiek tejto práce a je skvelé že zmenila svoj pohľad na vec,“ hovorí profesor Tilly. „Osobne si myslím, že oplodnenie týchto vajíčok je možné. Nevidím žiadne prekážky prečo by sa to nemohlo uskutočniť už tento rok,“ dodáva. Podľa doktorky Telferovej je dôležité poukázať na fakt, že je možné vziať kmeňové bunky z ľudských vaječníkov a tým využiť potenciál pre zachovanie ľudskej plodnosti. Dodáva, že ona a jej tím majú všetky potrebné etické povolenia na lokálnej úrovni a čaká ich už len proces schvaľovania Orgánom pre dohľad nad oplodňovaním a embryológiou (HFEA).

Preklad: Ingrid Tonhauserová

Foto: martinfrost.ws
Zdroj: independent.co.uk, pôvodný článok nájdete tu

    



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