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Vedci sa usilujú o implantovanie embryí nesúcich genetický materiál od troch rodičov


 | 16.5.2011 | komentárov: 636

V nasledujúcich riadkoch prinášame preklad článku z internetového vydania britského denníka Guardian. Pôvodný článok, ako aj reakcia poslanca Evana Harrisa, sa venuje otázke testovania dvoch nových techník, ktoré by sa mohli používať pri umelom oplodňovaní. Ide o prenos materského vretenovitého vlákna a pro-jadrový prenos, ktoré použitím malého množstva DNA tretích osôb umožňujú predchádzať vzniku dedičných chorôb. Diskutované metódy by mohli priniesť zmeny v právnej úprave umelého oplodnenia platnej vo Veľkej Británii Human Ferilisation and Emryology Act naposledy novelizovanej v roku 2008.

Obrazok

„Malý“ kúsok DNA tretej osoby môže zamedziť prenosu dedičných chorôb, tvrdia výskumníci. Dve experimentálne techniky, ktoré chcú vedci skúsiť, sú prenos materského vretenovitého vlákna a pro-jadrový prenos.

Vedúce výskumné organizácie a skupiny pacientov požadujú od vlády Veľkej Británie zmenu zákonov, ktoré by umožnili vedcom implantovať do ženského tela embryá obsahujúce genetický materiál od troch rozličných rodičov.Tento postup bol doteraz testovaný iba v laboratórnych podmienkach a zväčša na živočíšnych embryách. Ak sa tento postup ukáže ako bezpečný a úspešný, môže zamedziť narodeniu niekoľkých stoviek detí s genetickými poruchami. Tieto postihnutia zahŕňajú slepotu, zlyhanie orgánov, svalové ochorenia, poruchy učenia a cukrovku. Veľa detí umiera na následky týchto genetických porúch, ktoré sú spôsobené mutáciami v mitochondriálnej DNA. Tá sa nachádza iba vo vajíčku matky.

Britskí vedci sa snažia nájsť spôsoby ako zabrániť prenosu dedičných chorôb, a tým predísť smrti alebo postihnutiu detí. Požiadavka na zmenu práva prichádza v liste adresovanom  Andrewi Lensleymu, ministrovi zdravotníctva. List od Welcome Trust, Academy of Medical Sciences, Medical Research Council a Mascular Dystrophy Campaign bol okrem iného   odoslaný po tom, ako skupina odborníkov publikovala kontrolnú správu zadanú min. zdravotníctva Lansleym týkajúcu sa bezpečnosti a účinnosti vedeckých postupov o ktoré sa doteraz pokúsili. Vedci preskúmali dve odlišné techniky, pričom obe zahŕňajú zmiešanie DNA troch rodičov s malým množstvom mitochondriálnej DNA z vajíčka darcu.

Jeden z autorov správy, ktorá bola momentálne predložená vláde, profesor Robin-Lovell-Badge, tvrdí: „Nejde ale o umelé oplodnenie troch rodičov (...)Nie je to termín, ktorý  by sme raz použili v tejto správe a nie je to termín, ktorý by sa mal používať.“„Je to maličký, nepatrný kúsok DNA. Neprenáša žiadnu z vlastností, okrem toho, že máte normálne fungujúce mitochondrie.“ Prínos DNA z vajíčka s normálnymi mitochondriami je nepatrný v porovnaní s DNA od dvoch hlavných rodičov.

Dr. Evan Harris, bývalý poslanec parlamentu za liberálnych demokratov, ktorý sa bližšie zaujímal o výskum embryí, to prirovnal k výmene batérií v laptope, ktorá nemá vplyv na informácie na pevnom disku.“

Kontrolná správa (http://www.hfea.gov.uk/docs/2011-04-18_Mitochondria_review_-_final_report.PDF) vypracovaná pod záštitou Human Fertilisation and Embryology Authority, zistil, že vedecké postupy sa javia byť bezpečné a účinné, ale odporučil uskutočniť tri ďalšie pokusy.

Niektorí ľudia sa zdajú byť váhaví a odmietaví v postoji k našej správe – čo sme tým vôbec nesledovali,“ povedal Lovell-Badge. „Chceli sme tým len zozbierať trochu viac informácií.“

Jedna z dvoch experimentálnych techník sa volá prenos materského vretenovitého vlákna  a zahŕňa odňatie genetického materiálu z neoplodneného vajíčka matky a jeho zlúčenie s vajíčkom darkyne, z ktorého bolo odňaté jadro. K oplodneniu partnerovou spermiou dochádza až následne. „Bolo to odskúšané na rôznych zvieratách a zdá sa to byť tak účinné ako aj bezpečné,“ povedal Lovell-Badge. Nebolo to však odskúšané s použitím ľudských vajíčok, čo by vedci radi videli.

Ďalšou metódou je pro-jadrový prenos, ktorý skúmali na Inštitúte Genetickej Medicíny na Univerzite v Newcastle (Institute of Genetic Medicine at Newcastle University). Táto metóda zahŕňa prenos oboch rodičovských DNA z oplodneného vajíčka do oplodneného vajíčka darkyne, ktoré má odňaté jadro, čo sa  úspešne zrealizovalo na myšiach začiatkom osemdesiatych rokov. V Newcastle to uskutočnili aj s použitím abnormálnych ľudských vajíčok. Lovell-Badge a jeho tím by rád videli tento pokus na oplodnených ľudských vajíčkach a taktiež na opiciach, aby si boli istí bezpečný výsledkom.

Ďalšie pokusy by nemali trvať viac ako rok. Vedci a skupiny pacientov v súčasnosti vyvíjajú nátlak na vládu, aby zvážila príslušné právne a etické otázky nato, aby sa vykonali potrebné zmeny právnej úpravy a experimenty mohli byť zavedené do klinickej praxe čo najskôr.

 Dodatok: Podľa názoru poslanca Evana Harrisa: „Vláda musí konať.“

Obyčajne, by každá nová liečba ako vyššie navrhované, ktorá používa oplodnené vajíčka alebo si vyžaduje umelé oplodnenie, musela byť schválená Úradom pre ľudské oplodňovanie a embryológiu (Human Fertilisation and Embryology Authority- HFEA) ako bezpečná a účinná, predtým než  sa vyskúša na dobrovoľníkoch. Bežnou požiadavkou by ďalej bolo, aby umelé oplodnenie bolo nevyhnutné a žiadané týmito pacientmi.

Problémom je, že v roku 2008, namiesto umožnenia HFEA robiť tieto rozhodnutia, parlament na naliehanie vtedajšej vlády, zakázal použitie vajíčka,  ktoré malo pridaný alebo pozmenený čo i len nepatrný kúsok genetického materiálu počas oplodňovania.

 Avšak, s vedomím, že týmto zúfalým rodinám sa bude dať čoskoro pomôcť, bolo do zákona pridané oprávnenie vlády vydávať nariadenia, ktoré jej umožňujú prijímať úpravy, ktoré  povoľujú HFEA udeliť licenciu na použitie tejto technológie, pričom nariadenie musí byť schválené zvlášť každou komorou parlamentu..

 Správa zverejnená HFEA tento týždeň (v polovici apríla pozn. prekl.)  potvrdzuje, čo skupiny vedcov, doktorov a pacientov už vedia. Nie je žiaden dôkaz, že tieto techniky sú nebezpečné a že je potrebné len obmedzené množstvo ďalších pokusov v laboratóriu a na zvieratách k preukázaniu, že procedúra je bezpečná.

 Tieto pokusy sa už začali a nebudú trvať dlho. Preto je neodkladné, aby vláda navrhla verejnú diskusiu ohľadom etickej stránky tohto postupu a  predložila, že návrh opatrení  do parlamentu.

 

Preklad: Štefan Lazorčák

 

Zdroj: http://www.guardian.co.uk/science/2011/apr/19/scientists-embryos-three-parents

Foto: bryanwcole.com


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